Table 1 The characterization of three multiplex PCR sets for the amplification of 14 microsatellite loci in 69 unrelated samples of European Robin.

From: First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity

Locus	Primer sequence (5′–3′)	Dye	Size range	A	H_o	H_E	F_IS	HWE	F(null)
ER24	F: TGTTGTCACTTTGCACCTGG	TAM	128–185	16	0.594	0.696	0.154*	0.0104	0.079
ER24	R: CTGCTTCACTCCTTGCACAG		128–185	16	0.594	0.696	0.154*	0.0104	0.079
ER40	F: GATTGCAGGGAACGCTGAC	FAM	190–217	10	0.696	0.799	0.136	0.4742	0.071
ER40	R: CAGGTGCTGCTGGAATGTTC		190–217	10	0.696	0.799	0.136	0.4742	0.071
ER28	F: TGGTTAATGAAGATGACGGCG	HEX	214–283	14	0.826	0.864	0.052	0.1114	0.021
ER28	R: AAGATCCCAGCCTCATTCCG		214–283	14	0.826	0.864	0.052	0.1114	0.021
ER21	F: ATGGATGCTGTGGGAGTCTC	ROX	225–273	13	0.913	0.889	− 0.020	0.4540	− 0.013
ER21	R: GTGATCACGACAGTCAGCAC		225–273	13	0.913	0.889	− 0.020	0.4540	− 0.013
ER38	F: CGCCTGTGTAAATCTCGCTG	FAM	283–358	12	0.797	0.799	0.010	0.3520	0.003
ER38	R: TTGTTCCAACCTAGCCTGGG		283–358	12	0.797	0.799	0.010	0.3520	0.003
ER15	F: TACCTCCTGCTTGCTGTGAG	HEX	299–415	21	0.913	0.908	0.002	0.7325	− 0.002
ER15	R: TCCCAACCAAGTCTCCAAGG		299–415	21	0.913	0.908	0.002	0.7325	− 0.002
ER32	F: CTCACCTGTGCTCTTTAAGGC	TAM	106–139	9	0.638	0.730	0.133	0.4697	0.066
ER32	R: TGCAGTTCTTCAGTTTGGTGG		106–139	9	0.638	0.730	0.133	0.4697	0.066
ER7	F: CAACTGGTCAGCAACACTCC	ROX	262–294	10	0.899	0.851	− 0.048	0.7983	− 0.028
ER7	R: TTGCAAGCTCAGGATCCTTG		262–294	10	0.899	0.851	− 0.048	0.7983	− 0.028
ER4	F: TCAGAGGTGTGGCAGAAAGG	FAM	204–292	17	0.696	0.850	0.188	0.0675	0.102
ER4	R: TTGTTCCTGCTGCTTTGGAC		204–292	17	0.696	0.850	0.188	0.0675	0.102
ER5	F: ACTCCCTACACTTGCCAAGG	FAM	308–428	14	0.826	0.842	0.026	0.7992	0.009
ER5	R: GTCTAAAGATGCCCAGAACCTG		308–428	14	0.826	0.842	0.026	0.7992	0.009
ER39	F: CGGTGAACAAGAGCAGAAGG	TAM	144–216	22	0.913	0.923	0.018	0.5100	0.004
ER39	R: CCTTCTTCACTGCAAGCTGG		144–216	22	0.913	0.923	0.018	0.5100	0.004
ER13	F: AGTCTCCTTGCTGCTCTGTG	ROX	180–256	19	0.913	0.885	− 0.025	0.2387	− 0.017
ER13	R: TGCCCTTTCTCTGACATGGG		180–256	19	0.913	0.885	− 0.025	0.2387	− 0.017
ER25	F: GAAAGTATCGTGGCAATGCAC	HEX	272–332	19	0.896	0.914	0.028	0.3522	0.010
ER25	R: TTCAGGTTCGACAAAGAGGC		272–332	19	0.896	0.914	0.028	0.3522	0.010
ER31	F: GAAATGGGAACTGTGCTGGC	FAM	298–344	26	0.754	0.935	0.201*	0.0000	0.106
ER31	R: CTGCAGGCTTACTTCAACCAG		298–344	26	0.754	0.935	0.201*	0.0000	0.106

Column headings are as follows: Size range—size of fragments obtained during PCR, including flanking region; A—number of alleles; H_O—observed heterozygosity; H_E—expected heterozygosity; F_IS—fixation index (*—F_IS value significant after Bonferroni correction, 200 randomizations, adjusted P-value = 0.00357), HWE—P-value of Hardy–Weinberg equilibrium exact test, F(null)—null allele frequency. Annealing temperature for all PCRs was 57 °C.

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Table 1 The characterization of three multiplex PCR sets for the amplification of 14 microsatellite loci in 69 unrelated samples of European Robin.

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