Figure 1
From: BTN2A2 protein negatively regulates T cells to ameliorate collagen-induced arthritis in mice
The expression pattern of BTN2A2 protein on immune cells. (A) Splenocytes from C57BL/6 mice that were freshly harvested were used for resting immune cells. To activate T cell, splenocytes were incubated with anti-CD3 antibody (1 μg/ml) for 3 days. To activate B cells, DCs, monocytes and macrophages, splenocytes were stimulated with LPS (5 μg/ml) for 3 days. The resting and activated immune cells were stained with anti-BTN2A2 or isotype antibody as well as anti-CD4, CD8, CD19, CD11c or F4/80 antibody to identify immune cells. The cells were analyzed for BTN2A2 protein expression by flow cytometry. Representative flow cytometric profiles, and (B) Statistical analysis for the expression levels BTN2A2 on immune cells. Data are presented as relative fluorescence intensity (RFI) for the cells stained with antiBTN2A2 antibody versus cells stained with isotype antibody. *P < 0.05 compared with isotype antibody. **P < 0.05 compared with resting cells. The data are representative of three independent experiments with similar results (n = 3 each experiment).
