Figure 2
From: BTN2A2 protein negatively regulates T cells to ameliorate collagen-induced arthritis in mice
The expression pattern of the putative BTN2A2 receptor on immune cells. Resting and activated immune cells were prepared as Fig. 1. The immune cells were incubated with biotinylated BTN2A2-Ig or control Ig protein for 4 h, followed by PE conjugating streptavidin and antibodies against CD4, CD8, CD11c or F4/80 or CD19. (A) Representative flow cytometric profiles and (B) statistical analysis showing the binding of BTN2A2-Ig or control Ig to resting and activated immune cells. Data are presented as the percentage of cell binding of BTN2A2-Ig versus control Ig. *P < 0.05 compared with control Ig. **P < 0.05 compared with resting cells. The data are representative of three independent experiments with similar results. (n = 3 each experiment).
