Figure 3
From: BTN2A2 protein negatively regulates T cells to ameliorate collagen-induced arthritis in mice
The effects of BTN2A2-Ig protein on murine T cell activation and proliferation in vitro. Splenocytes from C57BL/6 mice were incubated on 96-well plate precoated with 0.5 μg/ml of anti-CD3 antibody and the indicated dose of BTN2A2-Ig or control Ig. The cells were analyzed for (A) the expression of CD69 18 h later and (B) the percentages of CD44loCD62Lhi naïve and CD44hiCD62Llo effective memory CD4 and CD8 T cells 72 h later, and (C) the expression of Ki67 by CD4 or CD8 positive T cells 72 h later. The data are representative of three independent experiments with similar results. (n = 3 each experiment) *P < 0.05, **P < 0.01 and ***P < 0.001, compared with control Ig.
