Figure 4
From: BTN2A2 protein negatively regulates T cells to ameliorate collagen-induced arthritis in mice
Administration of BTN2A2-Ig alleviates CIA in mice. Male 6–8-week-old DBA/1J mice were immunized with 200 μg of bovine collagen II emulsified in CFA on day 0, and rechallenged with bovine collagen II emulsified in IFA on 21 days. When clinical scores were reached 8, The mice were injected i.p. with 15 μg BTN2A2-Ig or control Ig protein two times per week for 4 weeks. CIA development was monitored over time. (A) A flowchart for the generation of CIA model mice, (B) CIA clinical scores, (C) representative images of hind paws from control Ig- or BTN2A2-Ig protein-treated CIA mice and untreated normal mice, and (D) statistical analysis of the thickness of hind paws. (E–H) The rear paws were harvested from the mice on day 84. (E,F) H&E staining of (E) the sections of metatarsophalangeal joint from control Ig- or BTN2A2-Ig-treated CIA mice, and (F) pathological scores are shown. (G,H) Safranin O-fast green staining of (G) representative hind paws, and (H) pathological scores are shown. The data are representative of three independent experiments (n = 6 each experiment each group) with similar results.
