Figure 5
From: BTN2A2 protein negatively regulates T cells to ameliorate collagen-induced arthritis in mice
BTN2A2-Ig inhibits T cell activation and proliferation in CIA mice. DBA/1J CIA mice were treated with BTN2A2-Ig or control Ig as in Fig. 4. The spleens were harvested 35 days later. Spleen cells were stained with anti-CD69 or isotype antibodies as well as anti-CD4, CD8 antibody to identify activated cells. The splenocytes were analyzed for (A,B) the expression of CD69 by CD4 and CD8 T cells, (C,D) the percentages of CD44loCD62Lhi naïve and CD44hiCD62Llo effector memory in CD4 and CD8 T cells, and T cell proliferation by (E,F) CFSE dilution assay and (G,H) Ki67 staining. (I,J) The splenocytes were also analyzed for the percentages of CD25+FoxP3+CD4+ Tregs. The data are expressed as the mean ± SD and representative of three independent experiments with similar results. (n = 6 each experiment each group) *P < 0.05, ***P < 0.001 compared with control-Ig.
