Figure 2 | Scientific Reports

Figure 2

From: Oxysterol binding protein-like 3 (OSBPL3) is a novel driver gene that promotes tumor growth in part through R-Ras/Akt signaling in gastric cancer

Figure 2

Effect of OSBPL3 on cell proliferation and tumor growth in GC. (a) MTT assays in GC cells (MKN45 and MKN74) transfected with OSBPL3-specific siRNAs. si: siRNA targeting OSBPL3. N = 9, error bars represent the mean ± SD. Student’s t-test. (b) Colony formation assays in GC cells (MKN45 and MKN74) transfected with OSBPL3-specific siRNAs. si: siRNA targeting OSBPL3. N = 3, error bars represent the mean ± SD. Student’s t-test. (c) Growth curve of tumors derived from 5.0 × 106 GC cells (transfected with OSBPL3-specific shRNA or control-shRNA) injected into xenograft mice. sh: shRNA targeting OSBPL3. N = 7, error bars represent the mean + SD. Student’s t-test. (d) The weights of tumors derived from 5.0 × 106 GC cells ((transfected with OSBPL3-specific shRNA or control-shRNA) injected into xenograft mice and representative images of the tumors. sh: shRNA targeting OSBPL3. N = 7, error bars represent the mean ± SD. Student’s t-test. (e) The percentage of Ki67-positive cells in tumors derived from 5.0 × 106 GC cells ((transfected with OSBPL3-specific shRNA or control-shRNA) injected into xenograft mice (left panel). N = 7, error bars represent the mean ± SD. Immunohistochemical analyses of OSBPL3 and Ki67 in a representative sample from xenograft mice (right panel). sh: shRNA targeting OSBPL3. Student’s t-test.

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