Table 1 Plant growth regulator (PGR) treatments and concentrations and supplement contents were applied by somatic embryogenesis. Note that the base medium was half-strength Litvay’s medium.
From: Somatic embryogenesis in mature zygotic embryos of Picea pungens
Step | PGR concentration (μM) | Supplement (g/L) |
|---|---|---|
Initiation | Low: BA 1.25 + 2,4-D 2.5 BA 2.5 + 2,4-D 5.0 Standard: BA 5.0 + 2,4-D 10.0 High: BA 7.5 + 2,4-D 12.5 BA 7.5 + 2,4-D 15.0 BA 10.0 + 2,4-D 15.0 BA 10.0 + 2,4-D 20.0 | Sucrose 10.0 Gelrite 4.0 Glutamine 0.5 |
Proliferation | Liquid: BA 3.75 + 2,4-D 6.25 | Sucrose 10.0 Glutamine 0.5 |
Solid: BA 7.5 + 2,4-D 12.5 | Sucrose 10.0 Gelrite 4.0 Glutamine 0.5 | |
Maturation | ABA 60.0 | Sucrose 60.0 Gelrite 6.0 Glutamine 0.5 |
Germinationa | Sucrose 20.0 Gelrite 6.0 Glutamine 0.5 |
