Figure 6
From: Immunomodulatory effects of thalidomide in an experimental brain death liver donor model
EMSA competition studies and supershift assays performed using nuclear extracts from hepatic tissue samples in the presence of specific oligonucleotides. Supershift assays were performed on nuclear extracts incubated in the absence and presence of antibodies against the p65, p50 and cRel subunits. The position of the specific NF-κB-binding complex (p50/p65) is indicated. The results are representative of three experiments. The raw pictures of the EMSA supershift assay are shown in Supplementary Fig. S2.
