Table 1 Primer sequences used for Real Time PCR assays.
From: Immunomodulatory effects of thalidomide in an experimental brain death liver donor model
Gene | Sense and antisense (5′–3′) | Product (pb) |
|---|---|---|
TNF-α | 5′ TGGCCCAGACCCTCACACTCA 3′ | 541 |
5′ GGCTCAGCCACTCCAGCTGC 3′ | ||
IL-1β | 5′ CCTTGTGCAAGTGTCTGAAGCAGC 3′ | 248 |
5′ GCCACAGCTTCTCCACAGCCA 3′ | ||
IL-6 | 5′ CCGGAGAGGAGACTTCACAGAGGA 3′ | 71 |
5′ AGCCTCCGACTTGTGAAGTGGTATA 3′ | ||
IL-10 | 5′ TCAGTCACATTTGTTTTCTGCAAA 3′ | 65 |
5′ CTGCAAAAGTGGAGCAGTCATT 3′ | ||
MHC class I | 5′ TTCCTGCTACCGTTCCTCAC 3′ | 65 |
5′ GGTGTGAGTCCACATACCCA 3′ | ||
MHC class II | 5′ TCAGTCACATTTGTTTTCTGCAAA 3′ | 65 |
5′ CTGCAAAAGTGGAGCAGTCATT 3′ | ||
NF-κB | 5′ ATCAAAGAGCTGGTGGAGGC 3′ | 188 |
5′ GAAGGCTGCCTGGATCACTT 3′ | ||
β-actin | 5′ AGGAGTACGATGAGTCCGGCCC 3′ | 70 |
5′ GCAGCTCAGTAACAGTCCGCCT 3′ |
