Table 1 An infectious potential of CGMMV in BYIs and the corresponding dark green surrounding tissues of early and late post-recovery stages, determined in biological assays.

From: New early phenotypic markers for cucumber green mottle mosaic virus disease in cucumbers exposed to fluctuating extreme temperatures

CGMMV inoculum source

Temp (°C)

Symptom emergence (dpi)

Recovery (dpi)

Symptom reemergence (dpi)

Sampling day (dpi)

Infected plants (ratio)

ELISA positive OD range

'Early' BYIs (5 discs, 3 mm/disc, 28 mg)

25

10

None

na

27

5/5

0.3–0.4

32

10

13

19

22

5/5

0.3–0.5

'Early' total associated DST (~ 75 cm2,1.4 gr)

25

10

None

na

27

3/5

0.3–0.5

32

10

13

19

22

4/5

0.3–0.8

'Late' BYIs (4 discs, 20.8 mg)

25

9

None

na

14

15/15

0.23–0.25

32

9

13

18

10

12/15

0.21–0.31

'Late' associated DST (4 discs, 20.2 mg)

25

9

None

na

14

7/15

0.23–0.25

32

9

13

18

10

8/15

0.20–0.31

  1. 'Early' early post-recovery stage, 'Late' late post-recovery stage, DST dark surrounding tissues, dpi days post inoculation, na not applicable, ELISA negative reference OD range was 0.06–0.09; in brackets, numbers of collected discs, total disk weights and sizes of discs or leaf area.
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