Figure 2

Collagen compartmentalizes the group of tumor cells that looks like a big mass of cells if one only looks at H&E images (A, C). (D) Shows how the cell migration direction in an invasive ductal adenocarcinoma (IDC) can be visualized using PPM while this information cannot be readily extracted by just looking at BF image of H&E stained tissue (C). (E) and (F) Show that the difference in fiber thickness doesn’t change the color and color is only related to the fiber orientation (all blue fibers with different thickness) diameters flowing from top left corner to the bottom right corner are depicted as green. (G) and (H) Show curvy fibers result in periodic changes and this can be used as a comparison between straight and curvy fibers. The tissue area represented in images (A) to (E) is 1384 × 1038 µm, the area in image (F) is 692 × 519 µm and in images (G) and (H) is 346 × 259 µm. Images shown are purposely not post-processed in order to represent what the pathologist would see looking through the eyepiece.