Figure 1
From: Identification of neutralising pembrolizumab anti-drug antibodies in patients with melanoma
Pembrolizumab drug level and anti-drug anti-bodies: assay overview and standard curves. (A) Schematic of (left) pembrolizumab drug level assay. Sandwich enzyme-linked immune-sorbent assay (ELISA) with capture antibody (purple) that binds patient serum-derived pembrolizumab (blue). Detection antibody (green) is conjugated to horseradish peroxidase (HRP; red star) facilitating colormetric quantitation. Pembrolizumab anti-drug anti-body (ADA) assay (right) was achieved by sandwich ELISA using commercial pembrolizumab capture antibody (blue) to detect patient serum-derived ADA (red). Detection antibody was commercial pembrolizumab (blue) conjugated to HRP (red star). (B) Performance of commercial standard curved for pembrolizumab drug level (left) and ADA (right) are shown.
