Figure 1

Qualitative results of microCT and histological analysis for WT vs. 5LOKO mice treated or not with ZL on skeletal phenotyping WT and 5LOKO aged female mice. Aged 129 Sv WT and 5LOKO female mice were treated with 0.9% saline solution (Vehice) or ZL (250ug/Kg), once a week, for 7 weeks. Mice were euthanized and femur and L5 vertebrae were collected for analysis by microCT (A), H&E (B) and Picro-thionin (Schmorl) staining for osteocytes lacunae (C). (C) Scale bar 100 µm; (D) scale bar 50 µm. (A) Three-dimensional representative images obtained with the CT-Vox software (Skyscan, Kontich, Belgium).