Figure 8

Schematic illustration of the experimental protocol. Briefly, BALB/c mice received either distilled water or Lactobacillus casei Shirota fermented beverage (LcSFB) every day. Ovalbumin (OVA) was given to all groups by subcutaneous (s.c.) injection on the 14th, 28th, and 42nd days. Moreover, the NCn, Ln, and Hn groups were treated with OVA via the intranasal route on the 28th, 29th, 30th, 52nd, 53rd, and 54th days. Airway hyperresponsiveness (AHR) was assessed in the NCn, Ln, and Hn groups on the 55th day. The mice were sacrificed on the 56th day, and the proliferation of splenocytes, percentages of regulatory T (Treg) and T helper (Th) lymphocytes in splenocytes, OVA‐specific antibodies, and cytokines were measured.