Figure 1

Cell populations differ between peripheral and lesional blood in patients with mycosis fungoides (MF). (a) Flow cytometry analysis of CD4⁺ and CD8⁺ T cells from 2, 5, and 10 μL peripheral blood for the detection of T cells in small amounts of blood. The number in the upper right corner represents the number of CD4⁺ and CD8⁺ T cells. Mean (+ standard deviation) numbers of CD4⁺ and CD8⁺ cells (n = 3) are shown in the bar graph (right). (b) Lesional blood was collected during a skin biopsy. Peripheral and lesional blood was obtained from the patient’s arm and erythematous lesion, respectively, on the same day. The collected blood cells and serum were used for the subsequent experiments. (c) Flow cytometry of CD4⁺ and CD8⁺ T cells obtained from 5 μL of peripheral and lesional blood. Numbers of CD4⁺ and CD8⁺ T cells in 5 μL of peripheral and lesional blood are shown (right, n = 4). (d) Representative mass cytometry analysis of peripheral and lesional blood from a patient with MF through viSNE analysis using Cytobank. The color of each dot represents the immune cell subset. (e) The proportion of each cell population in peripheral and lesional blood obtained from patients with MF (n = 5) quantified by mass cytometry. A paired t-test was used for the statistical analysis. * P < 0.05.