Figure 3

TCR repertoire of lesional blood is skewed, and expression of genes differs between lesional and peripheral blood. (a) Gene expression analysis through RNA-seq in CD4⁺CD45RO⁺ T cells of peripheral and lesional blood from 3 patients (Cases 12, 16, and 17). Scatter plots show the expression values of every annotated gene. Blue and red dots indicate significant upregulation of CD4⁺CD45RO⁺ T cells in peripheral and lesional blood, respectively. (b) Ten top-ranked terms from the Jensen Diseases library of Enrichr for genes upregulated in CD4⁺CD45RO⁺ T cells of lesional blood. (c) Circos plots of frequencies of Vα and Jα gene usage and combinations of productive sequences in CD4⁺CD45RO⁺ T cells of peripheral and lesional blood. The band widths represents the frequency of each VJ pair. (d) Diversity of the TCR repertoire in CD4⁺CD45RO⁺ T cells of peripheral and lesional blood was evaluated using the Shannon–Weaver Index. (e) RNA-seq in CD8⁺CD45RO⁺ T cells of peripheral and lesional blood from 3 patients (Cases 4, 18, and 19). (f) Ten top-ranked gene ontology terms of biologic processes for genes upregulated in CD8⁺CD45RO⁺ T cells of lesional blood. (g) Circos plots of CD8⁺CD45RO⁺ T cells of peripheral and lesional blood. (h) Shannon–Weaver Index of CD8⁺CD45RO⁺ T cells of peripheral and lesional blood.