Figure 4

CD8⁺CD45RO⁺ TCR repertoire differs depending on the stage of MF. (a,c,e) Hematoxylin and eosin (HE) staining and immunofluorescence (IF) staining of 3 types of skin lesions (erythema, plaque, and tumor) from Case 10. CD8 (green) and CD45RO (red) are shown in the immunofluorescence staining image. Collagen is stained green, and red blood cells are stained red. Scale bar = 100 μm. (b,d,f,g) Circos plots of frequencies of Vα and Jα gene usage and combinations for CD8⁺CD45RO⁺ T cells in lesional blood from erythema, plaque, and tumor stages. The Circos plot at the bottom (g) is of cells obtained from peripheral blood. (h–l) Erythema and plaque of the same skin lesion from case 15. Same experiment as shown in panels (a–g).