Figure 2
From: A bimolecular fluorescence complementation flow cytometry screen for membrane protein interactions
Immunoblot analysis of the various BiFC complexes obtained from cell lysates. Samples were applied in the following order on the blots (a and b): (M) Marker, molecular weight in kDa, (1) YFPN − AQP0 + YFPC − AQP0, (2) YFPN − AQP0 + YFPC − CaM, (3) YFPN − AQP0∆ + YFPC − CaM, (4) YFP − AQP0, (5) YFPN − AQP0, (6) YFPC − CaM, (7) non-transformed S. cerevisiae cells. Monoclonal antibodies against YFPC (a) or YFPN (b) were used. The immunoblot confirms similar expression levels of the BiFC components. The bands corresponding to the BiFC fragments are indicated by arrows with the expected molecular weight and the part of the BiFC complex detected by the antibody is highlighted in bold.
