Figure 2

Analytical performance of PHASIFY MAX and ENRICH extraction kits. (a) cfDNA recovery of PHASIFY MAX (black bars) was assessed and compared against QCNA (white bars). Four or 10 ng of a synthetic 145 bp dsDNA fragment were spiked into 1 mL healthy human plasma, then processed by both extraction kits. The detection of the recovered cfDNA was performed using Taqman-based probe and primers specific for the 145 bp dsDNA sequence and quantified by ddPCR using a Bio-rad QX200 and QuantaSoft (version 1.7.4; www.bio-rad.com) (b) Agilent Bioanalyzer 2100 data demonstrates the ability of both PHASIFY MAX (blue) and QCNA (red) kits to recover all DNA fragment sizes from plasma samples spiked with DNA ladder (50, 75, 100, 150, 200, 300 bp), with PHASIFY-extracted samples obtaining higher peak amplitudes for each fragment size compared to QCNA. (c) Contrived samples of 1 ng 145 bp dsDNA spiked into 1 mL of plasma were processed with PHASIFY MAX and PHASIFY ENRICH (n = 3 each). Both kits achieved similar recovery of the 145 bp fragments as detected by ddPCR. (d) Plasma spiked with DNA ladder (500 – 10 k bp) were processed with either PHASIFY MAX (red) or PHASIFY ENRICH (blue) extraction kits and the resulting samples were analyzed with an Agilent Bioanalyzer. PHASIFY MAX extracted all fragment sizes contained in the DNA ladder while ENRICH removes larger DNA fragments (> 500 bp).