Figure 3 | Scientific Reports

Figure 3

From: Activators of alpha synuclein expression identified by reporter cell line-based high throughput drug screen

Figure 3

LUC assay and LUC RT-qPCR in the screening (A1) and control cell line (A6) for activators of α-syn. (A) Signal fold-change in the LUC assay of A1 and A6 cell lines and (B) expression fold changes of LUC mRNA measured with RT-qPCR. LUC signal change was determined by normalizing six replicates of treated cells to DMSO. RT-qPCR data were normalized to three housekeeping genes and DMSO control, in triplicates, respectively. Boxplot diagrams represent 5–95 percentile. Dotted lines in (A) depicts threshold of four-fold standard deviation of DMSO control. Compounds were applied at a final concentration of 25 µM (Clo 12.5 µM) for 24 h. VPA was used as a positive control at a concentration of 600 µM. ****p = 0.0001.

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