Figure 6
In-lysate and purified RNA Smart-3SEQ library prep accurately detect DEGs compared to TruSeq. Differentially expressed genes list for calmidazolium and fludrocortisone treated dermal fibroblast from in lysate and purified RNA were compared to Illumina TruSeq as a gold standard. True positives were identified as those genes in Smart3-SEQ methods with FDR < 0.1 and abs(log2FC) > 1. (a) Receiver Operating Curve (ROC) and (b) Precision-Recall curves (PR) were measured. Area under the curve (AUC) for each performance measurement is indicated in the figure.
