Table 1 Sequence of primer used for detection of mRNA.

From: Effect of chitosan on blood profile, inflammatory cytokines by activating TLR4/NF-κB signaling pathway in intestine of heat stressed mice

Primer name

Forward (5′–3′)

Reverse (5′–3′)

TNF-α

GGACTAGCCAGGAGGGAGAACAG

GCCAGTGAGTGAAAGGGACAGAAC

IL-10

TTCGCTGATGATGCTTAGTTT

ACAGGCAATGACGAAATA

TLR4

CACAGAAGAGGCAAGGCGACAG

GAATGACCCTGACTGGCACTAACC

P-65

GTCCCCGTGCCCTCTGTCTAAG

ACTGTTCCTGGTCCTGTGTAGCC

HSP 70

GTGGTGAACGACGGCGACAAG

GCCTCAGCGATCTCCTTCATCTTC

Claudin2

TCTGCCCTGTACCCCAACTG

CCCAGGAAGACGGGCTTT

Occludin

ATGGTGAAGGTCGGAGTGAAC

CTCGCTCCTGGAAGATGGT

β-actin

TATGCTCTCCCTCACGCCATCC

GTCACGCACGATTTCCCTCTCAG

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