Figure 4
The morphologies of late endosomes/lysosomes in VE cells of E5.5 embryos. Localisation of endocytic organelle markers, RAB7 (red) and Lamp2 (green) in wild-type (a–d) (n = 3) and Atp6v0c mutant embryos (e–h) (n = 7). Localisation of syntaxin7 (Stx7, red) and Lamp2 (green) in wild-type (i–l) (n = 11) and Atp6v0c mutant embryos (m–p) (n = 3). VE cells were labelled with GATA6 (blue). Large apical vacuoles labelled by Lamp2 were well developed in the wild-type VE cells, whereas Lamp2 signal was dispersed and the spherical structures were not observed in mutant embryo. Syntaxin7 co-localised with Lamp2 and was also enriched in the apical region of wild-type VE cells. The apical localisation pattern of syntaxin7 was also disturbed in mutant GATA6-positive VE cells. Scale bars are indicated in each panel.
