Figure 2
From: Modular fluorescent nanoparticle DNA probes for detection of peptides and proteins
PEGylation of carboxylate-modified FluoSpheres. (A) Zeta potential measurements for carboxylated FluoSpheres activated with NHS/EDC and reacted with mPEG-amine, mPEG-OH, or buffer only. Data are means ± standard deviation of three measurements per sample for one of two independent experiments, analyzed in duplicate. (B) Zeta potential measurements for carboxylated FluoSpheres activated with NHS/EDC and reacted with increasing concentrations of mPEG-amine. Data are means ± standard deviation of one experiment, analyzed in triplicate. Table lists theoretical distances between PEG molecules at each concentration. (C–E) FluoSpheres were conjugated with PEGs of indicated molecular weights, and (C) zeta potentials, (D) PDIs, and (E) hydrodynamic diameters were determined. Shown are means ± standard deviation of three measurements per sample, n = 2–3.
