Figure 4 | Scientific Reports

Figure 4

From: Leu-to-Phe substitution at prM146 decreases the growth ability of Zika virus and partially reduces its pathogenicity in mice

Figure 4

Recovery of Vero cell-adapted rNIID123 EPRV and in vitro growth of the point mutant rNIID123 prMF146L. (a) Focus phenotypes of rNIID123 EPRV #1 after one (P1) to four (P4) passages in Vero cells. (b) Partial amino acid sequences of the prM region in rNIID123 EPRV #1 P1, P4, and PRVABC59. Numbers indicate positions in the ZIKV prM protein. (c) Schematic representation of the point mutant rNIID123 prMF146L. (d) Focus phenotypes of rNIID123 and rNIID123 prMF146L in Vero cells. (e) Growth curves of rNIID123 EPRV P4 and rNIID123 prMF146L in Vero cells. Cells were inoculated with the viruses at an MOI of 0.01 ffu/cell. Means ± standard deviations of three independent experiments are shown.

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