Table 1 Metagenome samples and their groupings in the AD according to experiment time and methane (CH₄) concentration up to Day 35 of digestion (highest methane production level).

Groups	Day	CH₄ (%)	pH	DNA purity and concentration			Reads after QC	No. of OTUs	SRA accession
Groups	Day	CH₄ (%)	pH	Sample ID	Purity (280/260)	Concentration (ng/µL)	Reads after QC	No. of OTUs	SRA accession
Group-I	0	0	5.43	R1	1.78	103.7	20,962,184	437	SRR12814733
Group-I	0	0	5.46	M3	1.82	91.7	21,439,832	690	SRR12814732
Group-II	2	21	5.44	O4	1.88	61	22,222,964	687	SRR12814725
				I5	1.88	77.7	18,879,852	706	SRR12814724
				M6	1.87	73.4	22,207,206	1058	SRR12814723
	7	34	5.56	O7	1.87	46	21,742,834	1073	SRR12814722
	7	34	5.56	I9	1.88	87.2	22,074,694	555	SRR12814721
Group-III	10	47.4	5.97	O10	1.87	48.6	21,769,978	1069	SRR12814720
				M11	1.86	86.1	20,872,498	1007	SRR12814719
				I12	1.88	100.5	23,074,762	667	SRR12814718
	27	58.2	6.87	O60	1.89	59.4	22,044,170	1295	SRR12814731
	27	58.2	6.87	O13	1.89	61.3	18,850,242	380	SRR12814730
Group-IV	34	71.4	6.99	O15	1.81	67.7	21,713,262	1109	SRR12814729
	34	71.4	6.99	I14	1.82	65.4	22,361,088	673	SRR12814728
	35	74.1	7.01	O16	1.81	112.3	22,044,170	1168	SRR12814727
	35	74.1	7.01	I17	1.81	53.6	21,394,606	1384	SRR12814726

*R1 = Raw cow dung, M3 = mixtures with raw cow dung and slurry from previous biogas plant as seed, O = Outlet samples, I = Input position samples, M = Middle position samples.

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