Figure 3

Demonstration of 8AQs synergy with chloroquine in vitro using a 20-day assay. Chloroquine, primaquine and tafenoquine were assayed in the 20-day assay format alone and in combination with chloroquine as indicated on the X-axis. Chloroquine was found slightly toxic at 10 μM, thus the 0.1 and 1 μM combinations with 8AQs are shown. Nigericin was ran in all assays as a positive and normalization control. EC50 shown indicates potency against hypnozoites. Bars represent the SEM of three independent experiments; the first two experiments were the average of two replicate wells for each concentration, the third experiment was the average of four replicate wells for each concentration.