Figure 4

Receiver-operator characteristic (ROC) analyses of screen data. ROC curves were calculated for all compounds from the Reference Compound Library, Malaria Box, Pathogen Box, and Stasis Box for which dose response (DR) data were collected as part of this report, area under curve (AUC) and 95% CI are indicated. (A) ROC curves of Prophylactic or Radical Cure activity against schizonts or hypnozoites for compounds exhibiting unambiguously active or inactive curves in DR assays, regardless of possible toxicity. (B) ROC curves of Prophylactic or Radical Cure activity against schizonts or hypnozoites for compounds exhibiting unambiguously active or inactive curves and no toxicity at 10 μM in DR assays. ROC curves were not calculated for Radical Cure activity against hypnozoites because no hits were found from SP assays. (C) ROC curves of compounds found toxic at 10 μM in Prophylactic and Radical Cure DR assays. (A–C) Red line indicates a random assay. (D) Table quantifying all compounds considered putative hits in SP assays which were found toxic at 10 μM in DR confirmation assays. Only 3 compounds with Prophylactic activity against schizonts exhibited a selectivity window in DR confirmation, all other compounds did not exhibit selective activity.