Figure 6

Cross-comparison of Single Point activity data from other Plasmodium species and lifecycle stages. (A) ROC curves showing predictive value of other datasets grouped by P. berghei liver stage, P. falciparum Stage I-III gametocytes, P. falciparum Stage IV–V gametocytes, P. cynomolgi asexual blood stage, P. falciparum asexual blood stage and hepatic toxicity. Non-vivax data was combined into a single number for each compound by using the highest inhibition value from the different assays which generated data for that species and stage; PvLS data was combined into a single number by using the highest inhibition value obtained against either hypnozoites or schizonts in either mode, and if any value was > 75%, that compound was deemed “active” (or, for hepatic toxicity, > 9% nuclei loss was deemed “toxic”). Area under curve (AUC) and 95% CI are indicated. (B–D) X axis is breakout of hypnozoite and schizont data by mode from ‘A,’ Y-axis is other species or stage activity data, Single Point test concentration is noted in legends. (B) Breakout of data P. berghei data from ‘A.’ (C) Breakout of P. falciparum gametocyte data from ‘A.’ (D) Breakout of P. cynomolgi and P. falciparum blood stage data from ‘A.’ Red lines indicate 75% activity cutoffs. MMV019266, a nonselective false positive, is noted in (B) and (C).