Table 1 Summary of sequence alignment and phylogenetic signals for individual genes and concatenated datasets. Bootstrap proportion (BP) and transfer bootstrap expectation (TBE) are shown as percentages for each clade; bold letters denote significant support (≥ 80%); N, not recovered in best ML tree; NA, not applicable. Sensitivity analyses were run with three additional lower heterobranchs (w/AA) or without Rhodopidae (wo/Rho) or Murchisonellidae (wo/Mur). See Supplementary Figs. S1 and S2.
Nuc rRNA | MtDNA | All (4 genes) | Sensitivity analyses | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
18S | 28S | (18S + 28S) | 16S | COI | (16S + COI) | w/AA | wo/Rho | wo/Mur | ||
Number of taxa | 47 | 50 | 51 | 50 | 49 | 51 | 52 | 55 | 50 | 50 |
Total length (MAFFT) | 2474 | 1640 | 4114 | 522 | 657 | 1179 | 5293 | 5787 | 5216 | 5232 |
Final alignment (Gblocks) | 1689 | 947 | 2636 | 336 | 657 | 993 | 3629 | 3618 | 3644 | 3628 |
Variable sites | 620 | 594 | 1214 | 223 | 417 | 640 | 1854 | 2025 | 1851 | 1799 |
Parsimony informative | 417 | 497 | 914 | 209 | 377 | 586 | 1500 | 1740 | 1497 | 1464 |
Clade, BP/TBE (%) | ||||||||||
Tjaernoeioidea nov | 100/100 | 100/100 | 100/100 | 97/94 | 100/100 | 98/90 | 100/100 | 100/100 | 100/100 | 100/100 |
Rhodopidae | 93/93 | 94/87 | 84/86 | 92/82 | (NA) | 74/59 | 90/87 | 94/93 | (NA) | 100/100 |
Murchisonellidae | (NA) | 100/100 | 100/100 | 96/81 | N/N | 93/90 | 100/100 | 100/100 | 100/100 | (NA) |
Allomorpha | (NA) | 74/77 | 68/79 | N/N | N/88 | 48/N | 96/98 | 88/96 | (NA) | (NA) |
Mesoneura nov | N/N | 69/74 | 54/72 | N/N | 77/93 | N/73 | 96/93 | 64/83 | 83/93 | 78/64 |
Euthyneura | N/N | 36/69 | 40/74 | N/N | 95/99 | 93/99 | 100/100 | 95/98 | 91/95 | 97/98 |
Tetratentaculata nov | N/N | N/N | 36/62 | N/N | 53/84 | N/74 | 97/96 | 49/74 | 89/92 | 63/81 |
