Figure 3
From: Cytosolic protein delivery using pH-responsive, charge-reversible lipid nanoparticles
Cytosolic delivery of NLS-(− 30)GFP using DOP-DEDA-based LNPs. (A) Confocal laser scanning microscopy (CLSM) observation of the cytosolic appearance of NLS-(− 30)GFP after treatment with NLS-(− 30)GFP alone (left), NLS-(− 30)GFP-LNP (1:10) (center) and (1:50) (right) for 6 h. NLS-(− 30)GFP concentration = 2.5 μM. Scale bar, 50 μm. (B) Percentages of cells bearing cytosolic NLS-(− 30)GFP signals. Results are presented as mean ± standard deviation (SD) (n = 3). n.s., not significant; ***P < 0.001, (one-way analysis of variance [ANOVA] followed by Tukey–Kramer’s honestly-significant difference test).
