Figure 5
From: Cytosolic protein delivery using pH-responsive, charge-reversible lipid nanoparticles
Time- and concentration-dependence of the cytosolic delivery of NLS-(− 30)GFP-LNPs. (A) CLSM observation of the cytosolic appearance of NLS-(− 30)GFP after treatment with NLS-(− 30)GFP-LNPs (1:10) (equivalent to 2.5 μM NLS-(− 30)GFP) for 1, 3, and 6 h. (C) CLSM observation of the cytosolic appearance of NLS-(− 30)GFP after treatment with NLS-(− 30)GFP-LNP (1:10) (NLS-(− 30)GFP concentration = 0.1, 0.5, 1.0, 2.5, 5.0 or 10 μM) for 6 h. (B, D) Percentages of cells bearing cytosolic NLS-(− 30)GFP signals. Results are presented as mean ± SD (n = 3). ***P < 0.001 (ANOVA followed by Tukey–Kramer’s honestly-significant difference test for (B)).
