Table 1 Physicochemical characterization of NLS-(− 30)GFP-LNP by mixing NLS-(− 30)GFP with lipids at various mass ratios.

From: Cytosolic protein delivery using pH-responsive, charge-reversible lipid nanoparticles

NLS-(− 30)GFP (μM)

Lipid (mM)

Mass ratio

Size (d.nm)

PdI

10.3

25

1:10

172 ± 12

0.156 ± 0.04

5.2

25

1:20

154 ± 32

0.134 ± 0.09

3.4

25

1:30

116 ± 20

0.164 ± 0.08

2.6

25

1:40

98 ± 8

0.154 ± 0.05

2.1

25

1:50

92 ± 11

0.126 ± 0.01

  1. The lipid mixture was composed of DOP-DEDA, DPPC, and cholesterol at a 45:10:45 molar ratio, and 1 mol% DMG-PEG5k was added.
  2. The mass ratios denote NLS-(− 30)GFP-LNP/total lipids (w/w). PdI = polydispersity index. Results are presented as the mean ± standard deviation (SD) of more than three independent experiments.
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