Figure 2
Over-expressed MSL2 in sex chromosomal aneuploidy. (A) Immunofluorescence of Drosophila polytene chromosomes from third instar larvae of metafemales (left panel) and MSL2-metafemales (right panel). The red channel is the signal from SXL and the green channel is the signal from antibodies of MSL complex components. DNA is stained with DAPI in blue. Scale bars, 5 μm. (B, C) Ratio distributions of gene expression in metafemale (XXX) and MSL2-metafemale compared with normal diploid. All genes are divided into X (B) and autosomes (C) according to their positions on chromosomes. The vertical red solid line represents the ratio 1.00 (no change), the vertical blue solid line represents the ratio 1.50 [the ratio of gene dosage effects (3/2)], and the vertical blue dashed line shows the ratio 0.67 [the ratio of inverse dosage effects (2/3)]. The frequencies were plotted in bins of 0.05. (D) Gene expression changes along Drosophila chromosomes between MSL2-metafemale and metafemale. Each chromosome is separated by a vertical line. Lowess smoothed log2 fold change (log2 ratio) were ordered at equidistance by their chromosomal position. Regions with up-regulated gene expression are shown in dark blue, while regions with down-regulated gene expression are shown in light blue.
