Figure 6
Embryo-FISH. (A–C) Embryo-FISH of candidate gene TER94. (A) TER94 RNA expression patterns in entire embryo. The genotype of the sample is shown in the horizontal axis above, and the development stage of the sample is shown in the left vertical axis. The red pseudo-color is the signal from probe and the green pseudo-color is the signal from nucleus. Scale bars, 80 μm. (B) TER94 RNA subcellular location patterns in wild type embryos. The image shown at the bottom right shows a locally enlarged subcellular localization pattern. Scale bars, 30 μm. (C) Relative fluorescence intensity of TER94 RNA signal in trisomy 2L and MSL2-trisomy 2L compared with normal diploid. The relative fluorescence intensity is determined by comparison with DAPI signal. The expression of wild-type embryos at each stage of development are set at 1. Asterisk denotes a p-value < 0.05 by two-tailed Student’s t tests. (D–E) Changes of transcript localization of ERp60 (D) and Hsc70-4 (E) in trisomy 2L and MSL2-trisomy 2L. Scale bars, (D) 30 μm; (E) 80 μm.
