Figure 1 | Scientific Reports

Figure 1

From: Integrated analysis of dysregulated microRNA and mRNA expression in intestinal epithelial cells following ethanol intoxication and burn injury

Figure 1

Small RNA sequencing analysis to identify differentially expressed microRNAs in small intestinal epithelial cells 1 day after alcohol and burn injury. (A) Volcano plot of differentially expressed microRNAs (p < 0.1) where each point on the graph represents an individual microRNA. Log2 fold change between ethanol burn and sham vehicle treated mice is plotted on the x-axis and the − log10 of the padj value is plotted on the y-axis. Overall, small RNA sequencing analysis identified 65 differentially expressed microRNAs (including 37 upregulated and 28 downregulated). (B) Heat map of fold change expression (log scale) of 17 differentially expressed miRNAs (DEMs) in each ethanol burn sample (EB1-5) relative to sham vehicle controls. Each row shows the fold change (log scale) of an individual DEM (padj < 0.1), ranging from downregulated (green) to upregulated (red) (C) KEGG pathway analysis of validated microRNA gene targets utilizing miRnet. Each bar represents a KEGG pathway, with the number of associated gene targets in parentheses, found to be significantly enriched (p value < 0.05) among the validated gene targets of the 17 DEMs outlined in (B).

Back to article page