Figure 5

Pathway enrichment analysis using integrated analysis of differentially expressed miRNAs and gene targets with correlated expression changes. Validated and predicted gene targets for each identified DEM were collected from miRNet, TargetScan, and miRDB databases. Gene target lists were then integrated with gene expression data from mRNA sequencing. Gene targets with significantly upregulated expression were extracted for each downregulated DEM, while significantly downregulated gene targets were extracted for each upregulated DEM. The resulting upregulated and downregulated gene targets were compiled separately, and KEGG pathway enrichment analysis was performed using G:profiler with g:SCS multiple testing correction. All significantly enriched (padj < 0.05) pathways are shown in the bar graph above.