Figure 5
From: Loading equine oocytes with cryoprotective agents captured with a finite element method model
Oocyte deformation (i.e., volume changes) and associated changes in the average intracellular CPA concentration during CPA-unloading, i.e. exposure of CPA loaded cells to an isotonic solution without CPAs. Simulations were done for oocytes equilibrated in different EG concentrations (0.75 mol L−1: green lines, 1.0 mol L−1: blue lines, 1.5 mol L−1: red lines); cell changes/deformation (A) as well as the intracellular CPA concentration (B) have been determined versus the exposure time exposure to isotonic medium. The solid/dashed lines represent responses as obtained using FEM modeling, while the dotted lines represent responses as obtained using the 2-parameter model.
