Figure 1
From: Distinct roles of haspin in stem cell division and male gametogenesis
Haspin knockout and overexpression. Occurrence of H3T3ph (images) and levels of haspin mRNA (histograms) in haspin-ΚΟ (a) and haspin-ΟΕ (b) cells, as detected by indirect immunofluorescence and RT-qPCR assays, respectively. (c) Western blots with similar samples. The antibodies used recognize H3T3ph, centromeric antigens (ACA), Aurora B and actin (a loading control), as indicated. For full length blots, see Supplemental Data III. (d) Distribution of H3T3ph and haspin-eGFP in transiently transfected E14 cells expressing different levels of the fusion protein. The bottom row shows transiently transfected haspin-KO cells expressing exogenous haspin. (e) Spatial distribution of H3K9me3 in haspin-KO and haspin-OE cells. Merges and different confocal sections (z1 and z2) are shown. (Ctrl) corresponds to control cells. In all images, DNA has been stained with TO-PRO 3. Scale bars, 5 μm. RT-qPCR data with one of the two KO clones (KO1) has also been presented in a previous publication20.
