Figure 2
From: Distinct roles of haspin in stem cell division and male gametogenesis
Effects of haspin dosage on cell division and growth. Localization of Aurora B during metaphase in haspin-KO (a) and haspin OE (b) cells, as detected by indirect immunofluorescence. Maximum projection profiles (top rows) and different confocal sections (z1–z3) are shown in each case. Merged images are also included, together with a contrasted blowup. Histograms display the distribution of Aurora B along mitotic chromosomes in different subsets of cells. The localization patterns of Aurora B are explained in more detail in Figure S4. (c) Effects of haspin knockdown or overexpression on spindle length and chromosome congression. The images depict metaphase cells stained with antibodies against α-tubulin and/or DNA. The boxplots display the length of the spindle, whereas histograms show the incidence of misaligned chromosomes in metaphase cells. (d) Cell cycle kinetics (histograms) and growth rate (line plots) of haspin-KO and haspin-OE cells. (Ctrl) corresponds to control cells. In all cases, DNA has been stained with TO-PRO 3. Scale bars, 5 μm. For quantitative details and statistical analysis, see Supplementary Data IΙ.
