Figure 3
From: Distinct roles of haspin in stem cell division and male gametogenesis
Effects of haspin dosage on differentiation. (a) Formation and growth of embryoid bodies with haspin-KO and haspin-OE cells, as visualized by phase-contrast microscopy. The average surface area of embryoid bodies (in μm2 × 103) is indicated. Scale bars, 50 μm. (b) Teratoma formation by haspin-KO and haspin-OE cells 8 weeks after injection into immunocompromised mice. The relative sizes of the tumors are shown on the images and the corresponding histograms. Boxplots display the weight (in grams) of the teratomas in each ensemble. (c) Histological profile of the teratomas. Representative paraffin sections stained with Hematoxylin/Eosin. All three germ layers are present. Visible in the specimens are squamous epithelium (ectoderm); respiratory epithelium (endoderm); neural rosettes or mature neural tissue (ectoderm); and blood vessels (mesoderm). Masses of cartilage and muscle tissue (mesoderm) can also be distinguished in the teratomas, which also contain neural glial cells (ectoderm). Scale bars, 100 μm.
