Figure 4
From: Distinct roles of haspin in stem cell division and male gametogenesis
Transcriptome analysis of haspin-KO and haspin-OE cells. (a) Volcano plots depicting the fold change and p-values of the deregulated genes that are common in the KO1/KO2 and the OE1/OE2 clones. The horizontal line corresponds to log10 ≥ 1.3 and the vertical line to log2 ≥ 1. The top 10 hits in each clone, as calculated from their Manhattan distance, are indicated in both plots. Genes involved in gametogenesis are green. (b) Correlation between the number of deregulated genes common to KO1/KO2 or OE1/OE2 clones and the total number of genes in each mouse chromosome, as assessed by the Fisher exact test. (c) Selected gametogenesis Gene Ontology categories that show statistically significant enrichment (p < 0.05), when deregulated genes common to KO1/KO2 and OE1/OE2 are analyzed. (d) Venn diagram displaying the number of genes affected in both haspin-KO and haspin-OE cells and heatmap showing the log2 Fold Change of the 40 genes that are common in all clones, irrespective of haspin content. Genes directly or indirectly involved in gametogenesis are in red. (e) Relative expression levels of Dazl and Scml2, as assessed by RT-qPCR.
