Figure 5

Abundance of haspin and H3T3ph in the mouse testis. (a) Schematic representation of the spermatogonial differentiation process (left) and the colonization of the seminiferous epithelium by spermatogenic cells (right). (b) A representative paraffin section of adult mouse testis stained with Hematoxylin/Eosin. The different cell types are indicated. Scale bar, 10 μm. (c) Relative levels of haspin mRNA in various tissues as assessed by RT-qPCR. (d) Western blotting (WB) experiments using the aH3T3ph, aPMM and aM4M8 antibodies (for details see text). The permutations of the PMM motif (H3T3phK4me₃, H3K4me₃R8me₂, H3T3phR8me₂) are shown graphically on top of the second panel. Total histones from immature (17-day old) and adult mouse testes were extracted by HCl and analyzed by SDS-PAGE/Coomassie brilliant blue (CBB) staining, as indicated. Portions of this material were used for western blotting. (M): molecular mass markers; (Total): unfractionated HCl extract; (S) supernatant after high speed centrifugation; (P): residue after centrifugation. The position of histone H3 is indicated by an arrowhead. For full length gels and blots, see Supplemental Data III.