Figure 7
From: Distinct roles of haspin in stem cell division and male gametogenesis
Identification of the PMM signature during the cycle of the seminiferous epithelium. (a) Frozen sections of 7-day old mouse testis and kidney stained with aPMM. Insets correspond to mitotic cells. Scale bars, 10 μm. (b) Frozen sections of adult mouse testis depicting seminiferous tubules at stages V–VIII and VIII–X stained with aPMM and aM4M8. Scale bars, 20 μm. (c) Competitive inhibition assays in stage V–VIII tubules using various synthetic peptides. The red circle in the stick diagram indicates the H3T3ph mark, while the blue circles the H3K4me3 and H3R8me2 modifications, respectively. Boxplots and scatterplots depict aPMM staining intensity in the presence of the histone H3 peptides. The results confirm the presence of H3K4me3R8me2 in early round spermatids. Scale bars, 20 μm. (d) Competitive inhibition assays in stage VIII–X tubules. Boxplots and scatterplots depict aPMM staining intensity in the presence of the synthetic peptides. The assays confirm the presence of H3T3ph-H3K4me3–H3R8me2 combinations in late round and elongating spermatids. Scale bars, 20 μm. For quantitative details see Supplementary Data IΙ.
