Figure 1
From: hSSB2 (NABP1) is required for the recruitment of RPA during the cellular response to DNA UV damage
hSSB2 level increases after UVB exposure. (a,b) Representative western blot for hSSB2 and \(\upgamma\) Tubulin for HeLa cells protein extracts 1 h after increasing doses of UVB (a) and at increasing repair time (h) after 200 J/m2 UVB (b). Average hSSB2 levels (± sem) over 3 independent experiments have been reported in the graphs below the blots (ns: non-significant; *P-value < 0.05; **P-value < 0.01; ***P-value < 0.001 in a One-Way ANOVA). (c) Immuno-histo chemistry staining (brown staining) for SSB2 (top panels) and DNA UV damage (CPD, bottom panels) of skin sections from 3 days old C57BL/6 mice not exposed to UV (left panels), 6 h (middle panels) and 24 h (right panels) post UVB exposure.
