Figure 6
In silico analysis of binding of dog CXCR4 with mUb or Ub∆GG. The coupling analysis of dog CXCR4 with mUb (A) or Ub∆GG (B) was carried out on the ClusPro 2.0 server. Dog CXCR4 is shown in pink and mUb or Ub∆GG in blue. Dark pink or dark blue indicate the interface area between dog CXCR4 with mUb or Ub∆GG, respectively. The coupling visualization was done with PyMOL v2.0 (C), and the value of the dissociation constant (Kd) at 37 °C was assessed from the data obtained with ClusPro and the PRODIGY server. No statistical differences were found between the molecular coupling between the dog CXCR4 with any version of ubiquitin. The graph shows the median + interquartile range obtained in the statistical analysis using the Mann–Whitney test.
