Figure 7

Concerted age-dependent regulation of nucleotide metabolism in BAT. Metabolic network representing nucleotide metabolism and integrating age-dependent regulation observed in metabolomic, proteomic and mRNA expression data analyses. Dashed line-boxes depict different metabolic branches of nucleotide metabolism: purine metabolism, pyrimidine metabolism and pentose phosphate pathway. Each oval represents a metabolite and each box represents a gene/protein. As shown in the legend, color-coding is provided for metabolites, genes and proteins enriched (red) or depleted (blue) during BAT-aging. Metabolites whose concentration was not affected by aging in BAT are shown in grey. Color-coding of metabolites is based on the log2 fold change (log2FC) values calculated in comparison to the young, 2.5-months control group (Supplementary Table S2). For each protein and gene, age-dependent regulation is represented by the same color-coding; genes whose mRNA was not changed during BAT-aging are depicted in white boxes. Proteomic changes are based on heavy/light ratios comparing young versus aged mice (Table 1).