Table 1 Age-dependent proteomic regulation of nucleotide metabolism enzymes. Table representing enzymes involved in (1) nutrient/ energy metabolism, (2) nucleotide metabolism and (3) B-vitamin metabolism identified by comparative 18O-labeled proteomic analysis of young and aged BAT (n = 5). Proteins were annotated based on their Uniprot accession number53. Identified proteins were clustered depending on their function in the metabolic pathway: (1) pyruvate metabolism, TCA cycle, glycolysis, amino acid metabolism; (2) purine and pyrimidine metabolism; (3) vitamin transport, vitamin B6 metabolism and nicotinamide metabolism. Proteins that were reduced in aged mice were identified based on heavy/light ratios (H/L ratio) < 1, while H/L ratios > 2.5 depicted age-enriched enzymes.
Cluster | Metabolic pathway | Protein name (Uniprot accession no.) | Gene symbol | RefSeq ID | Proteomic H/L ratio |
|---|---|---|---|---|---|
Energy metabolism | Pyruvate metabolism | Acyl-CoA Synthetase Short Chain Family Member 2 (A2AQN4) | Acss2 | NM_019811 | 0.88 |
Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (Q8BH04) | Pck2 | NM_028994 | 0.76 | ||
Dihydrolipoamide S-Acetyltransferase (Q8BMF4) | Dlat | NM_145614 | 0.95 | ||
Acetyl-CoA Acetyltransferase 2 (Q8CAY6) | Acat2 | NM_009338 | 0.76 | ||
Acetyl-CoA Acetyltransferase 1 (Q8QZT1) | Acat1 | NM_144784 | 0.94 | ||
TCA Cycle | ATP Citrate Lyase (Q91V92) | Acly | NM_134037 | 0.96 | |
Isocitrate Dehydrogenase 1 (O88844) | Idh1 | NM_001111320 | 0.84 | ||
Glycolysis | Phosphoglycerate Mutase 2 (O70250) | Pgam2 | NM_018870 | 4.12 | |
Hexokinase 1 (G3UVV4) | Hk1 | NM_001146100 | 2.98 | ||
Amino acid metabolism | Branched Chain Keto Acid Dehydrogenase E1 Subunit Alpha (Q3U3J1) | Bckdha | NM_007533.5 | 0.89 | |
Branched Chain Amino Acid Transaminase 2 (D3Z7C8) | Bcat2 | NM_001243052 | 0.97 | ||
Nucleotide metabolism | Purine metabolism | Multifunctional protein ADE2 (Q9DCL9) | Paics | NM_001356971 | 0.52 |
Phosphoribosylformylglycinamidine synthase (Q5SUR0) | Pfas | NM_001159519 | 0.54 | ||
Phosphoglucomutase-2 (Q7TSV4) | Pgm2 | NM_025700 | 0.70 | ||
Phosphoribosyl pyrophosphate synthetase 1-like 3 (G3UXL2) | Prps1l3 | NM_001037746 | 0.75 | ||
ADP-sugar pyrophosphatase (Nudix Hydrolase 5) (Q9JKX6) | Nudt5 | NM_016918 | 0.80 | ||
Nucleoside Diphosphate Kinase 3 (Q9WV85) | Nme3 | NM_019730 | 0.38 | ||
Nucleoside Diphosphate Kinase 1 (P15532) | Nme1 | NM_008704 | 0.43 | ||
Inosine-5'-monophosphate dehydrogenase 2 (P24547) | Impdh2 | NM_001378921 | 0.90 | ||
Phosphodiesterase 8B (P15532) | Pde8b | NM_001170669 | 0.11 | ||
Phosphodiesterase 4D (F6QFD1) | Pde4d | NM_011056 | 0.57 | ||
AMP deaminase 3 (O08739) | Ampd3 | NM_001276301 | 0.83 | ||
Adenosine kinase (P55264) | Adk | NM_134079 | 0.93 | ||
Hypoxanthine Phosphoribosyltransferase 1 (P00493) | Hprt | NM_013556 | 0.59 | ||
Pyrimidine metabolism | Cytidine/Uridine Monophosphate Kinase 1 (Q9DBP5) | Cmpk1 | NM_025647 | 0.83 | |
Vitamin metabolism | Vitamin transport | Apolipoprotein A1 (Q00623) | Apoa1 | NM_009692 | 0.66 |
Apolipoprotein A4 (P06728) | Apoa4 | NM_007468 | 0.45 | ||
Vitamin B6 metabolism | Pyridoxamine 5'-Phosphate Oxidase (Q91XF0) | Pnpo | NM_146026 | 0.70 | |
Nicotinamide metabolism | NAD Kinase 2, Mitochondrial (Q8C5H8) | Nadk2 | NM_001159637 | 0.56 | |
Nicotinate Phosphoribosyltransferase (Q8CC86) | Naprt | NM_172607 | 0.90 |
