Figure 4 | Scientific Reports

Figure 4

From: Computer-aided engineering of a branching sucrase for the glucodiversification of a tetrasaccharide precursor of S. flexneri antigenic oligosaccharides

Figure 4

(A, C, E): Absorbance at 540 nm determined after the reducing sugar “ON/OFF” assay using crude enzyme extracts of the 49 mutants co-produced with either tig (Tf16, light salmon) or dnaK, dnaJ, grpE, groES, groEL chaperone proteins (KJE8, dark cyan) in presence of 100 g L−1 sucrose during 70 h incubation. The parental enzyme BRS-B Δ2 is indicated as a reference. Dashed lines indicate absorbance level of the inactive mutant BRS-B Δ2 E709Q. (B, D, F) boxplot representing the absorbance data. Parental enzyme BRS-B Δ2 was excluded from the analysis. Data points (diamonds) pinpoint the 16 mutants selected for further evaluation.

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